A flexible, open-access workflow to facilitate the identification of cell clones that have desirable CRISPR-Cas9-induced gene edits.
This only needs to be done once. To run GenEditID, first download and install these dependencies using bioconda. Here are some platform specific instructions for MacOS and for Windows 10.
curl -O https://repo.anaconda.com/miniconda/Miniconda3-latest-MacOSX-x86_64.sh
sh Miniconda3-latest-MacOSX-x86_64.sh
curl -O https://repo.anaconda.com/miniconda/Miniconda3-latest-Linux-x86_64.sh
sh Miniconda3-latest-Linux-x86_64.sh
conda config --add channels defaults
conda config --add channels bioconda
conda config --add channels conda-forge
geneditid
.
conda create -n geneditid python=3.8 pear seqkit git-lfs
To activate this environment, use conda activate geneditid
.
To deactivate an active environment, use conda deactivate
.
To see a list of all of your environments, run conda env list
.
To remove this environment, run conda remove -n geneditid --all
cd ~
conda activate geneditid
git lfs install
git clone https://github.com/GenEditID/GenEditID.git
cd GenEditID/
./scripts/setup_geneditid.sh
Due to large reference genomes files within the repository, the cloning step takes can take a while. Please wait till it completes before moving forward, thanks for your patience.
The active environment you are currently using is shown in parentheses () or brackets [] at the beginning of your command prompt.
(geneditid) $
if not, activate it using conda activate geneditid
before running the next steps.