GenEditID

A flexible, open-access workflow to facilitate the identification of cell clones that have desirable CRISPR-Cas9-induced gene edits.

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Step 4: Run ampli_count

Detailed information about the reference genome files

The Reference Genome (release 95): Homo_sapiens.GRCh38.dna.toplevel.fa.gz is stored in GenEditID/data/reference/ and has been compressed using bgzip. If you wish to work with another reference, please download, uncompress, and re-compress it with bgzip utility from samtools htslib-1.10.2

gunzip Homo_sapiens.GRCh38.dna.toplevel.fa.gz
bgzip < Homo_sapiens.GRCh38.dna.toplevel.fa > Homo_sapiens.GRCh38.dna.toplevel.fa.gz

:house: Back to the main manual

GenEditID Manual: detailed steps